purified recombinant human egf  (PeproTech)

Journal: PLoS ONE

Article Title: Modification of Pulsed Electric Field Conditions Results in Distinct Activation Profiles of Platelet-Rich Plasma

doi: 10.1371/journal.pone.0160933

Figure Lengend Snippet: Differential effects of SMLEF bipolar pulses, SMHEF pulses and thrombin on PRP activation, growth factor release, and cell proliferation.

Article Snippet: Growth factor-dependent cell proliferation was confirmed by addition of purified recombinant human EGF (Lonza, Portsmouth, NH, USA).

Techniques: Activation Assay

Journal: PLoS ONE

Article Title: Modification of Pulsed Electric Field Conditions Results in Distinct Activation Profiles of Platelet-Rich Plasma

doi: 10.1371/journal.pone.0160933

Figure Lengend Snippet: PRP, treated as described in the Methods, was centrifuged, the supernatant recovered and assayed for pro- and anti-angiogenic factors by ELISA and for stimulation of cell proliferation using serum-starved epithelial cells (MCF10A). Cell proliferation in response to the plasma supernatants of unactivated or activated PRP (panel E) is normalized to that obtained with supernatants of unactivated PRP. Purified recombinant human EGF 100 ng/mL added to serum-free media increased cell proliferation 1.75-fold relative to media alone (data not shown). Results shown are means ± SEM, n = 5. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Growth factor-dependent cell proliferation was confirmed by addition of purified recombinant human EGF (Lonza, Portsmouth, NH, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Purification, Recombinant